|
KMID : 1123920160300040250
|
|
Korean Journal of Oriental Physiology and Pathology
2016 Volume.30 No. 4 p.250 ~ p.256
|
|
|
Antioxidant Effect of Viola mandshurica W. Becker on the High Fat Diet-Induced Renal Oxidative Stress
|
|
Choi Mi-Hye
Park In-Sik
|
|
|
Abstract
|
|
|
|
The prevalence of renal disease is increased with the overweight and obesity. High fat diet-associated oxidative stress increases production of reactive oxygen species (ROS) and induces apoptosis. There are two types of antioxidant defense mechanisms for oxidative stress. One is the enzyme defense mechanism by antioxidant enzymes such as superoxide dismutase (SOD), and catalase (CAT). The other is non-enzyme defense mechanism by signaling molecules such as nuclear factor-like 2 (Nrf-2), HO-1. In this study, we induced obesity in mice with high fat diet for six weeks and thereafter administered orally Viola mandshurica for 4 weeks. V. mandshurica is known to clear heat, detoxify and cool blood, and subside a swelling effect. In the V. mandshurica administered group, the immunoreactive signal of the Tunel staining was weaker than that of obesity group. Proapoptotic Bax, caspase 3 immunoreactives of the V. mandshurica administered group was lower than those of obesity group, whereas anti-apoptotic Bcl-2 immunoreactity was higher in the V. mandshurica administered group. Antioxidant enzyme mechanism such as superoxide dismutase 2 (SOD2), catalase (CAT) immunoreactives of the V. mandshurica administered group and Antioxidant non-enzyme mechanism such as Nuclear factor-like 2 (Nrf2), Heme Oxygenase 1 (HO-1) immunoreactives of the V. mandshurica administered group was higher than those of obesity group. These results demonstrate that V. mandshurica had the antioxidant and anti-apoptosis effects on obese mice.
|
|
|
KEYWORD
|
|
|
Viola mandshurica W. Becker, Antioxidant effect, Apoptosis, High fat diet
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|
|